It has also been used to perform cell contact assay to test the biocompatibility of high density electrode arrays.
Pc12 cell line price.
Culture medium rpmi 1640 2mm glutamine 10 horse serum 5 foetal bovine serum fbs.
Place culture vessels in incubator at 37Âșc subculture when cell density reaches between 2 4 x 10 6 viable cells ml.
It has been used in research of hypoxia where acute hypoxia induces exocytosis and prolonged hypoxia can induce excessive exocytosis.
Eleven stable rearranged marker chromosomes are noted as well as unassignable chromosomes in addition to the majority of autosomes that are trisomic.
Using fluorescent cis parinaric acid and tbars lipid peroxidation was shown to be increased in the presence of trace amounts of free ferrous ion in pc12 cells.
Maintaining healthy cells is the key to experimental success and reproducible research results.
This increase in lipid peroxidation was enhanced by ethanol in a dose dependent manner and also correlated with loss of cell viability as.
Studies on the intoxication pathway of tetanus toxin in the rat pheochromocytoma pc12 cells line.
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The cell line is aneuploid female modal number 64 range 52 to 68 with chromosome counts in the near triploid range.
To give you confidence in the health of your cells every step of the way we ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health.
Binding internalization and inhibition of acetylcholine release.
Sandberg k berry c rogers tb.
J biol chem 1989.
Free radical generation is hypothesized to be the cause of alcohol induced tissue injury.
The pc12 cell line has been used to get more information about diseases of the brain.
Pc12 cells were used to find which prion protein fragments caused neuronal dysfunction.
Pc12 cells harbor a mutation of the max gene and do not express max protein hopewell and ziff 1995 presaging the discovery that human max is a hereditary human pcc susceptibility gene burnichon et al 2012b.
Seed flask 5 x 10 5 to 1 x 10 6 viable cells ml or use subcultivation ratio of 1 2 to 1 4.
Cell lines can be generated using several strategies.
One strategy is the culture and cloning of tumor cells examples of which include the pc 12 cell line derived from a pheochromocytoma the b16 c3 line derived from a murine melanoma the imr 32 line derived from a human neuroblastoma and the c6 cell line cloned from a rat glial tumor.