P53 Status Prostate Cancer Cell Lines

P53 gene structure and chromosome 17p alleles were studied in the three human prostate cancer cell lines lncap du 145 and pc 3.

P53 status prostate cancer cell lines.

One strain lncap gw had a mutation in one of two alleles at position 273 arg. Many different types of cancer show a high incidence of tp53 mutations leading to the expression of mutant p53 proteins. P53 gene structure and chromosome 17p alleles were studied in the three human prostate cancer cell lines lncap du 145 and pc 3. This leads to a de facto p53 loss enabling cancer cells to escape p53 dependent apoptosis in favor of mitotic cell division.

Our laboratory has two separate culture lines of the lncap human prostate cancer cells. Cell lines with a wt p53 gene. Using a system in which p53 function was conditionally restored to p53. We report an unexpected non canonical role of caspase 8 in the nucleus of cancer cells.

Four tables are presented for each type of cancer. Berglind h pawitan y kato s ishioka c and soussi t. Although no genetic alteration is found in the p53 gene of these cell lines it is possible that. One strain lncap gw had a mutation in one of two alleles at position 273 arg his.

A percent cell proliferation was determined by the wst 1 assay and b apoptosis was measured by annexin v staining by flow cytometry using a bd lsrfortessa instrument. We assessed mi 219 a small molecule inhibitor of mdm2 with improved pharmacokinetics over nutlin 3 for sensitization of prostate cancer cells to radiotherapy and androgen deprivation therapy a. One variable that may affect the radiosensitivity of prostate tumor cells is their p53 status because this gene controls radiation induced cell cycle arrest apoptosis and the repair of dna damage. Lapc 4 p53 r175h and vcap p53 r248w cells were treated with dmso or peitc for 24 h.

Peitc inhibits cell proliferation induces apoptosis and reactivates p53 mutants in prostate cancer cell lines. Analysis of p53 mutation status in human cancer cell lines. Our laboratory has two separate culture lines of the lncap human prostate cancer cells. A paradigm for cell line cross contamination.

Upon g2 m checkpoint activation caspase 8 cleaves the de ubiquitinase usp28 inhibiting its ability to stabilize p53. Understanding the functions of mutant p53 will help in the development of new therapeutic. External beam radiation therapy is an effective therapy for localized prostate cancer although failures occur at high rates. Our laboratory has two separate culture lines of the lncap human.

Increased murine double minute 2 mdm2 expression independent of p53 status is associated with increased cancer specific mortality for men with prostate cancer treated with radiotherapy. There is growing evidence that these mutant p53s have both lost wild type p53 tumor suppressor activity and gained functions that help to contribute to malignant progression.