P Egfr Antibody Cell Signaling

Mir 146a treatment decreased whereas its inhibitor increased protein levels of p egfr p erk1 2 p p38 and not significantly p akt.

P egfr antibody cell signaling.

1993 cell 73. Mutation of either of these serines results in upregulated egfr tyrosine autophosphorylation 10. 92 100 based on 22 pubmed citations. Rabbit recombinant monoclonal egfr phospho y1068 antibody ep774y.

Epidermal growth factor receptor proto oncogene c erbb 1 receptor tyrosine protein kinase erbb 1 egfr human. P egfr antibody 12a3 is a mouse monoclonal igg 1 provided at 50 µg 0 5 ml. A431 whole cell lysate 100ng ml egf for 2 5 minutes lane 4. P egfr supplied by cell signaling technology inc used in various techniques.

Raised against an egfr phosphopeptide of human origin. Mutation of either of these serines results in upregulated egfr tyrosine autophosphorylation 10. Hela egf 5 mins c. Recommended for detection of tyr 845 phosphorylated egfr of mouse rat and human origin by wb and ip.

Egfr carboxy terminal residues ser1046 and ser1047 are phosphorylated by cam kinase ii. Quantitation of activated signaling molecules. Phosphorylation of egfr at specific serine and threonine residues attenuates egfr kinase activity. Monoclonal antibody is produced by immunizing animals with a fusion protein containing the cytoplasmic domain of human egf receptor.

Egfr antibody a 10 is a high quality monoclonal egfr antibody also designated her1 antibody or erbb1 antibody suitable for the detection of the egfr protein of mouse rat and human origin. Top 3 egf signaling must haves. 1999 trends pharmacol sci 20 408 12. Non cross reactive with the non phosphorylated egfr nor with unrelated tyrosine phosphorylated proteins.

A431 whole cell lysate 100ng ml egf for 5 minutes. A431 whole cell lysate 100ng ml egf for 1 minute lane 3. Cited in 75 publication s. Hela egf 10 mins akt also known as protein kinase b pkb is a serine threonine specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism apoptosis cell proliferation transcription and cell migration.

Zero bias scores article reviews protocol conditions and more. Egfr antibody a 10 is available as both the non conjugated anti egfr antibody form as well as multiple conjugated forms of anti egfr antibody. Antibodies to beta tubulin or beta actin were used as loading controls and for semi quantitation. Independently reviewed in 14.

1999 curr opin cell biol 11 184 9. Egfr carboxy terminal residues ser1046 and ser1047 are phosphorylated by cam kinase ii. Validated in wb ihc flow cyt dot icc if and tested in mouse human. Akt pser473 antibody arg51558 a.