Neb s luna universal probe one step rt qpcr kit offers exceptional sensitivity reproducibility and rt qpcr performance rt qpcr targeting human gapdh was performed using the luna universal probe one step rt qpcr kit over an 8 log range of input template concentrations 1 μg 0 1 pg jurkat total rna with 8 replicates at each. Cdna synthesis and pcr amplification steps are performed in a single reaction using gene specific primers resulting in a streamlined rt pcr protocol. The onetaq one step rt pcr kit offers sensitive and robust end point detection of rna templates.
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