Normal Lung Fibroblast Cell Line

It is an autocrine growth factor as well as a mitogen for astrocytes schwann cells and fibroblasts.

Normal lung fibroblast cell line.

Selected product human pulmonary fibroblasts hpf. Cell pellet in rnalater 1 000 000 cells. Hiv 1 hepatitis b and hepatitis c are not detected for all donors and or cell lots. This is a normal diploid human cell line with 46 xy karyotype.

The modal chromosome number was 46 occurring in 70 of cells. Jacobs the cell line was derived from the human lung tissue of a 14 week old male fetus aborted from a 27 year old woman. Some researcher use human normal lung fibroblasts hel 299 and mrc 5 cells while others use normal cell lines that don t belong to lung tissue. For example 6 thio guanine 6 tg and 8 azaguanine 8 ag are cytotoxic to cell having functional hgprt.

Passage normal lung fibroblast cells when culture has reached approximately 80 to 90 confluence and are actively proliferating. Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation. The cell line isolated by leonard hayflick in the 1960s has been used extensively in scientific research with applications ranging from developing important theories in molecular biology and aging to the production of most human virus vaccines. Both x and y chromosomes were normal.

Wi 38 is a diploid human cell line composed of fibroblasts derived from lung tissue of a 3 month gestation aborted female fetus. The contributions from this cell line towards human virus vaccine production have been credited with avoiding disease in o. The rate of polyploidy was 3 6. This protein interacts with the egf tgf alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines.

Specially characterized normal human diploid fibroblast cultures this collection includes the imr 90 female and imr 91 male human fetal lung fibroblast lines which were developed expressly for the nia aging cell repository and are available at low intermediate and high population doublings. Warm complete growth medium to 37 c prior to use with the cells. Initiated in september 1966 by j. Chromosome frequency distribution 50 cells.

This hgprt gene encodes for the hgprt enzyme which is responsible for the conversion of the purines into nucleotides toxic purine analog such as nucleobase guanine. Human fetal lung fibroblast cells mrc 5 line the mrc 5 cell line is commonly utilized in vaccine development as a transfection host in virology research and for in vitro cytotoxicity testing. Cryopreserved clonetics tm normal human lung fibroblasts cells are guaranteed through 15 population doublings they are tested for negative von willebrand factor expression factor viii cytokeratins 18 and 19 and alpha smooth muscle actin all cells test negative for mycoplasma bacteria yeast and fungi.