Normal Human Fibroblast Cell Line

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Normal human fibroblast cell line.

Bladder cardiac dermal gingival lung airway prostate scleral uterine and vas deferens. I am using bj cell line normal human fibroblasts but unfortunately their efficiency is very poor and they take very long to propagate. Cryopreserved clonetics tm normal human lung fibroblasts cells are guaranteed through 15 population doublings they are tested for negative von willebrand factor expression factor viii cytokeratins 18 and 19 and alpha smooth muscle actin all cells test negative for mycoplasma bacteria yeast and fungi. Initiated in september 1966 by j.

Cell line origin skin. Cwr r1ca human prostate cancer cell line. Our normal human fibroblasts can also be used for drug screening. Jacobs the cell line was derived from the human lung tissue of a 14 week old male fetus aborted from a 27 year old woman.

Make sure that the caps of all components are tight thaw the components of fibroblast growth kit low serum atcc pcs 201 041 just prior to adding them to the fibroblast basal medium atcc pcs 201 030. Primary normal human dermal fibroblasts nhdf are isolated form the dermis of juvenile foreskin or adult skin from different locations like the face the breasts the abdomen and the thighs. Hiv 1 hepatitis b and hepatitis c are. Lifeline normal human fibroblasts provide an ideal cell system to study wound healing toxicology cancer or basic cell biology in various organs including skin lung bladder and the reproductive systems.

This protein interacts with the egf tgf alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. The cells are cryopreserved in their first passage to ensure the highest viability and plating efficiency. The cell line isolated by leonard hayflick in the 1960s has been used extensively in scientific research with applications ranging from developing important theories in molecular biology and aging to the production of most human virus vaccines. Atcc normal adult human primary dermal fibroblasts when grown in fibroblast basal media supplemented with fibroblast growth kit components provide an ideal cell system to propagate dermal fibroblasts in either serum free or low serum conditions.

Wi 38 is a diploid human cell line composed of fibroblasts derived from lung tissue of a 3 month gestation aborted female fetus. This encoded protein is associated with a psoriasis like skin phenotype. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma fibrosarcoma fibrosis xeroderma pigmentosum and histiocytoma. Fluorescence digital image gallery human fetal lung fibroblast cells mrc 5 line the mrc 5 cell line is commonly utilized in vaccine development as a transfection host in virology research and for in vitro cytotoxicity testing.

They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma fibrosarcoma fibrosis xeroderma. Primary normal human dermal fibroblasts nhdf are isolated from the dermis of juvenile foreskin and are provided in a cryopreserved format.